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Ki-67 antibody (AA 2969-3119)

This anti-Ki-67 antibody is a Mouse Monoclonal antibody detecting Ki-67 in IHC, WB, ICC and IP. Suitable for Rat.
Catalog No. ABIN7427540

Quick Overview for Ki-67 antibody (AA 2969-3119) (ABIN7427540)

Target

See all Ki-67 (MKI67) Antibodies
Ki-67 (MKI67) (Antigen Identified By Monoclonal Antibody Ki-67 (MKI67))

Reactivity

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  • 39
  • 25
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Rat

Host

  • 100
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Mouse

Clonality

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  • 86
Monoclonal

Conjugate

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This Ki-67 antibody is un-conjugated

Application

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Immunohistochemistry (IHC), Western Blotting (WB), Immunocytochemistry (ICC), Immunoprecipitation (IP)
  • Binding Specificity

    • 62
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    AA 2969-3119

    Purpose

    Monoclonal Antibody to Ki-67 Protein (Ki-67)

    Specificity

    The antibody is a mouse monoclonal antibody raised against Ki-67. It has been selected for its ability to recognize Ki-67 in immunohistochemical staining and western blotting.

    Purification

    Protein A + Protein G affinity chromatography

    Immunogen

    Recombinant Ki-67 Protein (Ki-67) corresdonding to Thr2969~Val3119 with N-terminal His Tag

    Isotype

    IgG
  • Application Notes

    Western blotting: 0.5-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

    Comment

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    1 mg/mL

    Buffer

    PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    4 °C,-20 °C

    Storage Comment

    Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

    Expiry Date

    24 months
  • Target

    Ki-67 (MKI67) (Antigen Identified By Monoclonal Antibody Ki-67 (MKI67))

    Alternative Name

    Ki-67

    Background

    MKI67, KIA, Ki67P, Antigen Identified By Monoclonal Antibody Ki-67

    UniProt

    D4A0Y6

    Pathways

    Glycosaminoglycan Metabolic Process
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