Iba1 antibody (AA 1-147)

Catalog No. ABIN7426496

The Mouse Monoclonal anti-Iba1 antibody has been validated for WB, IHC, IP and ICC. It is suitable to detect Iba1 in samples from Human.

Quick Overview for Iba1 antibody (AA 1-147) (ABIN7426496)

Target: Iba1 (IBA1) (Ionized Calcium-binding Adapter Molecule 1 (IBA1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Iba1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone: D1

Product Details anti-Iba1 Antibody

Binding Specificity: AA 1-147

Purpose: Monoclonal Antibody to Ionized Calcium-binding Adapter Molecule 1 (IBA1)

Specificity: The antibody is a mouse monoclonal antibody raised against AIF1. It has been selected for its ability to recognize AIF1 in immunohistochemical staining and western blotting.

Cross-Reactivity: Pig

Purification: Protein A + Protein G affinity chromatography

Immunogen: Recombinant Ionized Calcium-binding Adapter Molecule 1 (IBA1) corresdonding to Met1~Pro147 with N-terminal His Tag

Isotype: IgG1 kappa

Application Details

Application Notes: Western blotting: 0.01-3 μg/mL,Immunohistochemistry: 5-30 μg/mL,Immunocytochemistry: 5-30 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for Iba1

Target: Iba1 (IBA1) (Ionized Calcium-binding Adapter Molecule 1 (IBA1))

Alternative Name: IBA1

Background: AIF1, IRT1, Daintain, Protein G1, Allograft Inflammatory Factor 1

UniProt: P55008

Pathways: Smooth Muscle Cell Migration