MANF antibody (AA 28-182)

Catalog No. ABIN7426499

This Mouse Monoclonal antibody specifically detects MANF in WB and IHC. It exhibits reactivity toward Human.

Quick Overview for MANF antibody (AA 28-182) (ABIN7426499)

Target: MANF (Mesencephalic Astrocyte-Derived Neurotrophic Factor (MANF))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MANF antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: C2

Product Details anti-MANF Antibody

Binding Specificity: AA 28-182

Purpose: Monoclonal Antibody to Mesencephalic Astrocyte Derived Neurotrophic Factor (MANF)

Specificity: The antibody is a mouse monoclonal antibody raised against MANF. It has been selected for its ability to recognize MANF in immunohistochemical staining and western blotting.

Purification: Protein A + Protein G affinity chromatography

Immunogen: Recombinant Mesencephalic Astrocyte Derived Neurotrophic Factor (MANF) corresdonding to Gly28~Leu182 with N-terminal His Tag

Isotype: IgG1 kappa

Application Details

Application Notes: Western blottin:g: 0.5-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for MANF

Target: MANF (Mesencephalic Astrocyte-Derived Neurotrophic Factor (MANF))

Alternative Name: MANF

Background: ARMET, ARP, Arginine Rich Mutated In Early Stage Tumors, Arginine-rich protein

UniProt: P55145