SIRT1 antibody (AA 244-498)

Catalog No. ABIN7426619

This Mouse Monoclonal antibody specifically detects SIRT1 in WB, IHC, IP and ICC. It exhibits reactivity toward Human.

Quick Overview for SIRT1 antibody (AA 244-498) (ABIN7426619)

Target: SIRT1 (Sirtuin 1 (SIRT1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SIRT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone: C2

Product Details anti-SIRT1 Antibody

Binding Specificity: AA 244-498

Purpose: Monoclonal Antibody to Sirtuin 1 (SIRT1)

Specificity: The antibody is a mouse monoclonal antibody raised against SIRT1. It has been selected for its ability to recognize SIRT1 in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse, Rat

Purification: Protein A + Protein G affinity chromatography

Immunogen: Recombinant Sirtuin 1 (SIRT1) corresdonding to Glu244~Ala498 with N-terminal His Tag

Isotype: IgG2b kappa

Application Details

Application Notes: Western blotting: 0.01-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for SIRT1

Target: SIRT1 (Sirtuin 1 (SIRT1))

Alternative Name: SIRT1

Background: SIR2L1, Silent Mating Type Information Regulation 2 Homolog 1, NAD-Dependent Deacetylase Sirtuin-1, SIR2-like protein 1, SirtT1 75 kDa fragment

UniProt: Q96EB6

Pathways: MAPK Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Carbohydrate Homeostasis, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus, Negative Regulation of intrinsic apoptotic Signaling