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IL12B antibody (AA 21-315)

The Rabbit Polyclonal anti-IL12B antibody has been validated for WB, IHC, IP and ICC. It is suitable to detect IL12B in samples from Chicken.
Catalog No. ABIN7430642

Quick Overview for IL12B antibody (AA 21-315) (ABIN7430642)

Target

See all IL12B Antibodies
IL12B (Interleukin 12 beta (IL12B))

Reactivity

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Chicken

Host

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Rabbit

Clonality

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Polyclonal

Conjugate

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This IL12B antibody is un-conjugated

Application

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Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)
  • Binding Specificity

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    AA 21-315

    Purpose

    Polyclonal Antibody to Interleukin 12B (IL12B)

    Specificity

    The antibody is a rabbit polyclonal antibody raised against IL12B. It has been selected for its ability to recognize IL12B in immunohistochemical staining and western blotting.

    Purification

    Antigen-specific affinity chromatography followed by Protein A affinity chromatography

    Immunogen

    Recombinant Interleukin 12B (IL12B) corresdonding to Gln21~Arg315 with N-terminal His Tag

    Isotype

    IgG
  • Application Notes

    Western blotting: 0.5-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

    Comment

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    500 μg/mL

    Buffer

    0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

    Preservative

    ProClin

    Precaution of Use

    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    4 °C,-20 °C

    Storage Comment

    Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

    Expiry Date

    24 months
  • Target

    IL12B (Interleukin 12 beta (IL12B))

    Alternative Name

    IL12B

    Background

    P40, IL12-B, CLMF2, NKSF2, Natural Killer Cell Stimulatory Factor 2, Cytotoxic Lymphocyte Maturation Factor 2, Interleukin 23, P40 Subunit

    UniProt

    Q6X0K9

    Pathways

    JAK-STAT Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation
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