IL-4 antibody (AA 26-147) (APC)

Catalog No. ABIN7436810

This Guinea Pig Polyclonal antibody specifically detects IL-4 in WB, IHC, IP and ICC. It exhibits reactivity toward Rabbit.

Quick Overview for IL-4 antibody (AA 26-147) (APC) (ABIN7436810)

Target: IL-4 (IL4) (Interleukin 4 (IL4))

Reactivity: Rabbit

Host: Guinea Pig

Clonality: Polyclonal

Conjugate: This IL-4 antibody is conjugated to APC

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-IL-4 Antibody (APC)

Binding Specificity: AA 26-147

Purpose: Polyclonal Antibody to Interleukin 4 (IL4)

Specificity: The antibody is a cavia polyclonal antibody raised against IL4. It has been selected for its ability to recognize IL4 in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Interleukin 4 (IL4) corresdonding to Arg26~Ser147 (Accession # Q9MZR8)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,Immunocytochemistry in formalin fixed cells: 5-20 μg/mL,Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL,Immunohistochemistry in paraffin section: 5-20 μg/mL,Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for IL-4

Target: IL-4 (IL4) (Interleukin 4 (IL4))

Alternative Name: Interleukin 4

Background: BSF1, BCGF1 ,B Cell Stimulatory Factor 1, Lymphocyte stimulatory factor 1, B Cell Growth Factor, Binetrakin, Pitrakinra

UniProt: Q9MZR8

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation