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CKM antibody (AA 11-367)

The Rabbit Polyclonal anti-CKM antibody has been validated for WB, IHC, IP and ICC. It is suitable to detect CKM in samples from Dog.
Catalog No. ABIN7431053

Quick Overview for CKM antibody (AA 11-367) (ABIN7431053)

Target

See all CKM Antibodies
CKM (Creatine Kinase, Muscle (CKM))

Reactivity

  • 128
  • 63
  • 46
  • 18
  • 12
  • 8
  • 6
  • 4
  • 4
  • 3
  • 3
  • 2
  • 1
  • 1
  • 1
Dog

Host

  • 131
  • 42
  • 13
  • 2
  • 1
Rabbit

Clonality

  • 138
  • 50
Polyclonal

Conjugate

  • 110
  • 26
  • 15
  • 8
  • 7
  • 5
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This CKM antibody is un-conjugated

Application

  • 148
  • 99
  • 66
  • 63
  • 53
  • 20
  • 17
  • 13
  • 13
  • 9
  • 9
  • 7
  • 7
  • 3
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)
  • Binding Specificity

    • 29
    • 15
    • 11
    • 8
    • 8
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    • 4
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    AA 11-367

    Purpose

    Polyclonal Antibody to Creatine Kinase, Muscle (CKM)

    Specificity

    The antibody is a rabbit polyclonal antibody raised against CKM. It has been selected for its ability to recognize CKM in immunohistochemical staining and western blotting.

    Cross-Reactivity

    Human, Mouse, Rat

    Purification

    Antigen-specific affinity chromatography followed by Protein A affinity chromatography

    Immunogen

    Recombinant Creatine Kinase, Muscle (CKM) corresdonding to Lys11~Leu367 with N-terminal His Tag

    Isotype

    IgG
  • Application Notes

    Western blotting: 0.01-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

    Comment

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    500 μg/mL

    Buffer

    PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    4 °C,-20 °C

    Storage Comment

    Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

    Expiry Date

    24 months
  • Target

    CKM (Creatine Kinase, Muscle (CKM))

    Alternative Name

    Creatine Kinase, Muscle

    Background

    CK-MM, CKMM, CK3, CK-M, MM-CK, M-CK, Creatine kinase M-type, N-terminally processed

    UniProt

    P05123
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