PKLR antibody (AA 114-323)

Catalog No. ABIN7431305

The Rabbit Polyclonal anti-PKLR antibody has been validated for WB, IHC, IP and ICC. It is suitable to detect PKLR in samples from Mouse.

Quick Overview for PKLR antibody (AA 114-323) (ABIN7431305)

Target: PKLR (Pyruvate Kinase, Liver and RBC (PKLR))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PKLR antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-PKLR Antibody

Binding Specificity: AA 114-323

Purpose: Polyclonal Antibody to Pyruvate kinase isozymes R/L (PKLR)

Specificity: The antibody is a rabbit polyclonal antibody raised against PKLR. It has been selected for its ability to recognize PKLR in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Pyruvate kinase isozymes R/L (PKLR) corresdonding to Ile114~Phe323 (Accession # P53657)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.01-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.45 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for PKLR

Target: PKLR (Pyruvate Kinase, Liver and RBC (PKLR))

Alternative Name: PKLR

Background: PK, PyK, PK1, PKL, Pyruvate Kinase Isozymes R/L, Glutamic--pyruvic transaminase 1, R-type/L-type pyruvate kinase, Red cell/liver pyruvate kinase

UniProt: P53657

Pathways: Ribonucleoside Biosynthetic Process