DDB2 antibody (AA 166-383)

Catalog No. ABIN7442507

The Rabbit Polyclonal anti-DDB2 antibody has been validated for WB, IHC, IP and ICC. It is suitable to detect DDB2 in samples from Human.

Quick Overview for DDB2 antibody (AA 166-383) (ABIN7442507)

Target: DDB2 (Damage-Specific DNA Binding Protein 2, 48kDa (DDB2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DDB2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-DDB2 Antibody

Binding Specificity: AA 166-383

Purpose: Polyclonal Antibody to Damage Specific DNA Binding Protein 2 (DDB2)

Specificity: The antibody is a rabbit polyclonal antibody raised against DDB2. It has been selected for its ability to recognize DDB2 in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse, Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Damage Specific DNA Binding Protein 2 (DDB2) corresdonding to Pro166~Met383 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 1-5 μg/mL,Immunocytochemistry in formalin fixed cells: 5-20 μg/mL,Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL,Immunohistochemistry in paraffin section: 5-20 μg/mL,Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for DDB2

Target: DDB2 (Damage-Specific DNA Binding Protein 2, 48kDa (DDB2))

Alternative Name: DDB2

Background: DDBB, UV-DDB2, Xeroderma Pigmentosum Group E Protein, UV-Damaged DNA-Binding Protein 2, DDB p48 Subunit, Damage-specific DNA-binding protein 2

UniProt: Q92466

Pathways: DNA Damage Repair