ACTN2 antibody

Catalog No. ABIN7431689

The Rabbit Polyclonal anti-ACTN2 antibody has been validated for WB, IHC, IP and ICC. It is suitable to detect ACTN2 in samples from Human.

Quick Overview for ACTN2 antibody (ABIN7431689)

Target: ACTN2 (Actinin, alpha 2 (ACTN2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ACTN2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-ACTN2 Antibody

Purpose: Polyclonal Antibody to Actinin Alpha 2 (ACTN2)

Specificity: The antibody is a rabbit polyclonal antibody raised against ACTN2. It has been selected for its ability to recognize ACTN2 in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Actinin Alpha 2 (ACTN2) corresdonding to N-terminal His Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.01-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for ACTN2

Target: ACTN2 (Actinin, alpha 2 (ACTN2))

Alternative Name: ACTN2

Background: Alpha-actinin skeletal muscle isoform 2, F-actin cross-linking protein

UniProt: P35609

Pathways: Cell-Cell Junction Organization, Skeletal Muscle Fiber Development, Negative Regulation of Transporter Activity