HNF4A antibody (AA 131-330)

Catalog No. ABIN7439672

The Rabbit Polyclonal anti-HNF4A antibody has been validated for WB, IHC, ICC and IP. It is suitable to detect HNF4A in samples from Human.

Quick Overview for HNF4A antibody (AA 131-330) (ABIN7439672)

Target: HNF4A (Hepatocyte Nuclear Factor 4, alpha (HNF4A))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HNF4A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Product Details anti-HNF4A Antibody

Binding Specificity: AA 131-330

Purpose: Polyclonal Antibody to Hepatocyte Nuclear Factor 4 Alpha (HNF4a)

Specificity: The antibody is a rabbit polyclonal antibody raised against HNF4a. It has been selected for its ability to recognize HNF4a in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse, Pig, Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Hepatocyte Nuclear Factor 4 Alpha (HNF4a) corresdonding to Gln131~Ser330 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.01-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for HNF4A

Target: HNF4A (Hepatocyte Nuclear Factor 4, alpha (HNF4A))

Alternative Name: HNF4a

Background: HNF4, HNF4a7, HNF4a8, HNF4a9, MODY, MODY1, NR2A1, NR2A21, TCF, TCF14, Nuclear Receptor Subfamily 2,Group A,Member 1, Transcription factor 14

UniProt: P41235

Pathways: AMPK Signaling, Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Carbohydrate Homeostasis, Cell-Cell Junction Organization, Regulation of Carbohydrate Metabolic Process