Nestin antibody (AA 178-399)

Catalog No. ABIN7431884

This Rabbit Polyclonal antibody specifically detects Nestin in WB, IHC, ICC and IP. It exhibits reactivity toward Human.

Quick Overview for Nestin antibody (AA 178-399) (ABIN7431884)

Target: Nestin (NES)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nestin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Product Details anti-Nestin Antibody

Binding Specificity: AA 178-399

Purpose: Polyclonal Antibody to Nestin (NES)

Specificity: The antibody is a rabbit polyclonal antibody raised against NES. It has been selected for its ability to recognize NES in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Nestin (NES) corresdonding to Leu178~Pro399 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-3 μg/mL,Immunohistochemistry: 5-30 μg/mL,Immunocytochemistry: 5-30 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for Nestin

Target: Nestin (NES)

Alternative Name: Nestin

UniProt: P48681