Nestin antibody (AA 197-462)

Catalog No. ABIN7439675

The Rabbit Polyclonal anti-Nestin antibody has been validated for WB, IHC, ICC, IP and FACS. It is suitable to detect Nestin in samples from Mouse.

Quick Overview for Nestin antibody (AA 197-462) (ABIN7439675)

Target: Nestin (NES)

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nestin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP), Flow Cytometry (FACS)

Product Details anti-Nestin Antibody

Binding Specificity: AA 197-462

Purpose: Polyclonal Antibody to Nestin (NES)

Specificity: The antibody is a rabbit polyclonal antibody raised against NES. It has been selected for its ability to recognize NES in immunohistochemical staining and western blotting.

Cross-Reactivity: Human

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Nestin (NES) corresdonding to Arg197~Pro462 (Accession # Q6P5H2) with N-terminal His and GST Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.01-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Flow cytometry:20 μg/mL,Optimal working dilutions must be determined by end user.,

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for Nestin

Target: Nestin (NES)

Alternative Name: Nestin

UniProt: Q6P5H2