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Thrombomodulin antibody (AA 279-493)

The Rabbit Polyclonal anti-Thrombomodulin antibody has been validated for WB, IHC, IP and ICC. It is suitable to detect Thrombomodulin in samples from Dog.
Catalog No. ABIN7431920

Quick Overview for Thrombomodulin antibody (AA 279-493) (ABIN7431920)

Target

See all Thrombomodulin (THBD) Antibodies
Thrombomodulin (THBD)

Reactivity

  • 131
  • 39
  • 22
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  • 1
Dog

Host

  • 76
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Rabbit

Clonality

  • 93
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Polyclonal

Conjugate

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This Thrombomodulin antibody is un-conjugated

Application

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Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)
  • Binding Specificity

    • 40
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    AA 279-493

    Purpose

    Polyclonal Antibody to Thrombomodulin (TM)

    Specificity

    The antibody is a rabbit polyclonal antibody raised against TM. It has been selected for its ability to recognize TM in immunohistochemical staining and western blotting.

    Purification

    Antigen-specific affinity chromatography followed by Protein A affinity chromatography

    Immunogen

    Recombinant Thrombomodulin (TM) corresdonding to Arg279~Ser493 with N-terminal His and GST Tag

    Isotype

    IgG
  • Application Notes

    Western blotting: 0.5-2 μg/mL,Immunocytochemistry in formalin fixed cells: 5-20 μg/mL,Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL,Immunohistochemistry in paraffin section: 5-20 μg/mL,Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL,Optimal working dilutions must be determined by end user.

    Comment

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    500 μg/mL

    Buffer

    0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

    Preservative

    ProClin

    Precaution of Use

    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    4 °C,-20 °C

    Storage Comment

    Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

    Expiry Date

    24 months
  • Target

    Thrombomodulin (THBD)

    Alternative Name

    Thrombomodulin

    Background

    CD141, THBD, BDCA3, THRM, Fetomodulin

    UniProt

    Q5W7P8
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