Caspase 3 antibody (AA 29-175)

Catalog No. ABIN7432241

This Rabbit Polyclonal antibody specifically detects Caspase 3 in WB, IHC, IP and ICC. It exhibits reactivity toward Rat.

Quick Overview for Caspase 3 antibody (AA 29-175) (ABIN7432241)

Target: Caspase 3 (CASP3)

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Caspase 3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-Caspase 3 Antibody

Binding Specificity: AA 29-175

Purpose: Polyclonal Antibody to Caspase 3 (CASP3)

Specificity: The antibody is a rabbit polyclonal antibody raised against CASP3. It has been selected for its ability to recognize CASP3 in immunohistochemical staining and western blotting.

Cross-Reactivity: Human, Mouse, Pig

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Caspase 3 (CASP3) corresdonding to Ser29~Asp175 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.01-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for Caspase 3

Target: Caspase 3 (CASP3)

Alternative Name: CASP3

Background: CPP32, CPP32B, SCA1, Apoptain, Yama, Apoptosis-Related Cysteine Peptidase, Cysteinyl Aspartate Specific Proteinases 3, SREBP cleavage activity 1

UniProt: P55213

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Sensory Perception of Sound, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation