Midkine antibody (AA 22-143)

Catalog No. ABIN7432268

The Rabbit Polyclonal anti-Midkine antibody has been validated for WB, IHC, IP and ICC. It is suitable to detect Midkine in samples from Human.

Quick Overview for Midkine antibody (AA 22-143) (ABIN7432268)

Target: Midkine (MDK) (Midkine (Neurite Growth-Promoting Factor 2) (MDK))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Midkine antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-Midkine Antibody

Binding Specificity: AA 22-143

Purpose: Polyclonal Antibody to Midkine (MK)

Specificity: The antibody is a rabbit polyclonal antibody raised against MK. It has been selected for its ability to recognize MK in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse, Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Midkine (MK) corresdonding to Ala22~Asp143 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for Midkine

Target: Midkine (MDK) (Midkine (Neurite Growth-Promoting Factor 2) (MDK))

Alternative Name: Midkine

Background: MDK, ARAP, NEGF2, Neurite Growth-Promoting Factor 2, Amphiregulin-associated protein, Midgestation and kidney protein, Neurite outgrowth-promoting factor 2

UniProt: P21741

Pathways: RTK Signaling, M Phase, Skeletal Muscle Fiber Development