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EGFR antibody (AA 319-484)

This anti-EGFR antibody is a Rabbit Polyclonal antibody detecting EGFR in WB, IHC, IP and ICC. Suitable for Mouse.
Catalog No. ABIN7432544

Quick Overview for EGFR antibody (AA 319-484) (ABIN7432544)

Target

See all EGFR Antibodies
EGFR (Epidermal Growth Factor Receptor (EGFR))

Reactivity

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Mouse

Host

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Rabbit

Clonality

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Polyclonal

Conjugate

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This EGFR antibody is un-conjugated

Application

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Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)
  • Binding Specificity

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    AA 319-484

    Purpose

    Polyclonal Antibody to Epidermal Growth Factor Receptor (EGFR)

    Specificity

    The antibody is a rabbit polyclonal antibody raised against EGFR. It has been selected for its ability to recognize EGFR in immunohistochemical staining and western blotting.

    Purification

    Antigen-specific affinity chromatography followed by Protein A affinity chromatography

    Immunogen

    Recombinant Epidermal Growth Factor Receptor (EGFR) corresdonding to Glu319~Pro484 (Accession # Q01279)

    Isotype

    IgG
  • Application Notes

    Western blotting: 0.01-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

    Comment

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    0.48 mg/mL

    Buffer

    PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    4 °C,-20 °C

    Storage Comment

    Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

    Expiry Date

    24 months
  • Target

    EGFR (Epidermal Growth Factor Receptor (EGFR))

    Alternative Name

    EGFR

    Background

    ErbB-1, ErbB1, ER1, ERB-B1, MENA, HER1, Erythroblastic Leukemia Viral(v-Erb-B)oncogene Homolog,Avian, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1

    UniProt

    Q01279

    Pathways

    NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation, S100 Proteins
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