Prothrombin antibody (AA 324-617)

Catalog No. ABIN7440125

The Rabbit Polyclonal anti-Prothrombin antibody has been validated for WB, IHC, IP and ICC. It is suitable to detect Prothrombin in samples from Rat.

Quick Overview for Prothrombin antibody (AA 324-617) (ABIN7440125)

Target: Prothrombin (F2) (Coagulation Factor II (thrombin) (F2))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Prothrombin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-Prothrombin Antibody

Binding Specificity: AA 324-617

Purpose: Polyclonal Antibody to Coagulation Factor II (F2)

Specificity: The antibody is a rabbit polyclonal antibody raised against F2. It has been selected for its ability to recognize F2 in immunohistochemical staining and western blotting.

Cross-Reactivity: Human

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Coagulation Factor II (F2) corresdonding to Thr324~Arg617 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,1:220-900Immunohistochemistry: 5-20 μg/mL,1:22-90Immunocytochemistry: 5-20 μg/mL,1:22-90Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.45 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for Prothrombin

Target: Prothrombin (F2) (Coagulation Factor II (thrombin) (F2))

Alternative Name: Coagulation Factor II

Background: FII, TM, PT, Thrombin, Prothrombin, Pro-Thrombin, Activation peptide fragment 1, Activation peptide fragment 2

UniProt: P18292

Pathways: Complement System, Peptide Hormone Metabolism, Regulation of G-Protein Coupled Receptor Protein Signaling