CRH antibody (AA 39-187)

Catalog No. ABIN7429703

This Rabbit Polyclonal antibody specifically detects CRH in WB, IHC, IP and ICC. It exhibits reactivity toward Rat.

Quick Overview for CRH antibody (AA 39-187) (ABIN7429703)

Target: CRH (Corticotropin Releasing Hormone (CRH))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CRH antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-CRH Antibody

Binding Specificity: AA 39-187

Purpose: Polyclonal Antibody to Corticotropin Releasing Factor (CRF)

Specificity: The antibody is a rabbit polyclonal antibody raised against CRF. It has been selected for its ability to recognize CRF in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Corticotropin Releasing Factor (CRF) corresdonding to Gln39~Lys187 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.01-3 μg/mL,Immunohistochemistry: 5-30 μg/mL,Immunocytochemistry: 5-30 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for CRH

Target: CRH (Corticotropin Releasing Hormone (CRH))

Alternative Name: Corticotropin Releasing Factor

Background: CRH, Corticotropin Releasing Hormone, Corticoliberin

UniProt: P01143

Pathways: Positive Regulation of Peptide Hormone Secretion, Hormone Activity, Negative Regulation of Hormone Secretion, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Feeding Behaviour