Prolactin antibody (AA 1-226)

Catalog No. ABIN7440458

The Rabbit Polyclonal anti-Prolactin antibody has been validated for WB, IHC, IP and ICC. It is suitable to detect Prolactin in samples from Mouse.

Quick Overview for Prolactin antibody (AA 1-226) (ABIN7440458)

Target: Prolactin (PRL)

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Prolactin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-Prolactin Antibody

Binding Specificity: AA 1-226

Purpose: Polyclonal Antibody to Prolactin (PRL)

Specificity: The antibody is a rabbit polyclonal antibody raised against PRL. It has been selected for its ability to recognize PRL in immunohistochemical staining and western blotting.

Cross-Reactivity: Pig

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Prolactin (PRL) corresdonding to Met1~Cys226

Isotype: IgG

Application Details

Application Notes: Western blotting: 1:50-400Immunocytochemistry in formalin fixed cells: 1:50-500Immunohistochemistry in formalin fixed frozen section: 1:50-500Immunohistochemistry in paraffin section: 1:10-100Enzyme-linked Immunosorbent Assay: 1:100-5000Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: PBS, pH 7.4, containing 0.01 % SKL, 1 mM DTT, 5 % Trehalose and Proclin300.

Preservative: Dithiothreitol (DTT), ProClin

Precaution of Use: This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for Prolactin

Target: Prolactin (PRL)

Alternative Name: Prolactin

Background: LTH, Luteotropic Hormone

UniProt: P06879

Pathways: JAK-STAT Signaling, Peptide Hormone Metabolism, Response to Growth Hormone Stimulus, Protein targeting to Nucleus