APAF1 antibody (AA 31-350)

Catalog No. ABIN7433081

This Rabbit Polyclonal antibody specifically detects APAF1 in WB, IHC, IP and ICC. It exhibits reactivity toward Human.

Quick Overview for APAF1 antibody (AA 31-350) (ABIN7433081)

Target: APAF1 (Apoptotic Peptidase Activating Factor 1 (APAF1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This APAF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-APAF1 Antibody

Binding Specificity: AA 31-350

Purpose: Polyclonal Antibody to Apoptotic Peptidase Activating Factor 1 (APAF1)

Specificity: The antibody is a rabbit polyclonal antibody raised against APAF1. It has been selected for its ability to recognize APAF1 in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Apoptotic Peptidase Activating Factor 1 (APAF1) corresdonding to Ser31~Phe350 (Accession # O14727)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.01-3 μg/mL,Immunohistochemistry: 5-30 μg/mL,Immunocytochemistry: 5-30 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for APAF1

Target: APAF1 (Apoptotic Peptidase Activating Factor 1 (APAF1))

Alternative Name: APAF1

Background: CED4, Apoptotic Protease Activating Factor

UniProt: O14727

Pathways: p53 Signaling, Apoptosis, Caspase Cascade in Apoptosis, Tube Formation, Positive Regulation of Endopeptidase Activity