ube3a antibody (AA 158-407)

Catalog No. ABIN7433114

The Rabbit Polyclonal anti-ube3a antibody has been validated for WB, IHC, IP and ICC. It is suitable to detect ube3a in samples from Human.

Quick Overview for ube3a antibody (AA 158-407) (ABIN7433114)

Target: ube3a (Ubiquitin Protein Ligase E3A (ube3a))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ube3a antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-ube3a Antibody

Binding Specificity: AA 158-407

Purpose: Polyclonal Antibody to Ubiquitin Protein Ligase E3A (UBE3A)

Specificity: The antibody is a rabbit polyclonal antibody raised against UBE3A. It has been selected for its ability to recognize UBE3A in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Ubiquitin Protein Ligase E3A (UBE3A) corresdonding to Ser158~Leu407 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 1-5 μg/mLImmunocytochemistry in formalin fixed cells: 5-20 μg/mLImmunohistochemistry in formalin fixed frozen section: 5-20 μg/mLImmunohistochemistry in paraffin section: 5-20 μg/mLEnzyme-linked Immunosorbent Assay: 0.05-2 μg/mLOptimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for ube3a

Target: ube3a (Ubiquitin Protein Ligase E3A (ube3a))

Alternative Name: UBE3A

Background: ANCR, AS, E6-AP, EPVE6AP, HPVE6A, Papilloma Virus E6-Associated,Angelman Syndrome, Oncogenic protein-associated protein E6-AP, Renal carcinoma antigen NY-REN-54

UniProt: Q05086

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway