NUP35 antibody (AA 132-310)

Catalog No. ABIN7433144

This anti-NUP35 antibody is a Rabbit Polyclonal antibody detecting NUP35 in WB, IHC, IP and ICC. Suitable for Human.

Quick Overview for NUP35 antibody (AA 132-310) (ABIN7433144)

Target: NUP35 (Nucleoporin 35kDa (NUP35))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NUP35 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-NUP35 Antibody

Binding Specificity: AA 132-310

Purpose: Polyclonal Antibody to Nucleoporin 35kDa (NUP35)

Specificity: The antibody is a rabbit polyclonal antibody raised against NUP35. It has been selected for its ability to recognize NUP35 in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Nucleoporin 35kDa (NUP35) corresdonding to Thr132~Lys310 (Accession # Q8NFH5)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,1:230-900Immunohistochemistry: 5-20 μg/mL,1:23-90Immunocytochemistry: 5-20 μg/mL,1:23-90Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.46 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for NUP35

Target: NUP35 (Nucleoporin 35kDa (NUP35))

Alternative Name: NUP35

Background: MP44, NP44, Mitotic phosphoprotein 44, Nuclear pore complex protein Nup53

UniProt: Q8NFH5