NG2 antibody (AA 1919-2258)

Catalog No. ABIN7437680

This Rabbit Polyclonal antibody specifically detects NG2 in WB, IHC, IP and ICC. It exhibits reactivity toward Rat.

Quick Overview for NG2 antibody (AA 1919-2258) (ABIN7437680)

Target: NG2 (MCSP) (Melanoma Associated Chondroitin Sulfate Proteoglycan (MCSP))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NG2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-NG2 Antibody

Binding Specificity: AA 1919-2258

Purpose: Polyclonal Antibody to Chondroitin Sulfate Proteoglycan 4 (CSPG4)

Specificity: The antibody is a rabbit polyclonal antibody raised against CSPG4. It has been selected for its ability to recognize CSPG4 in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Chondroitin Sulfate Proteoglycan 4 (CSPG4) corresdonding to Gly1919~Lys2258 (Accession # Q00657) with N-terminal His and GST Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for NG2

Target: NG2 (MCSP) (Melanoma Associated Chondroitin Sulfate Proteoglycan (MCSP))

Alternative Name: CSPG4

Background: CSPG4, MSK16, HMW-MAA, MCSP, MCSPG, MEL-CSPG, NG2, Chondroitin Sulfate Proteoglycan 4, Melanoma chondroitin sulfate proteoglycan

UniProt: Q00657

Pathways: Glycosaminoglycan Metabolic Process