MAP4K5 antibody (AA 613-842)

Catalog No. ABIN7439981

The Rabbit Polyclonal anti-MAP4K5 antibody has been validated for WB, ELISA, ICC, IHC (p) and IHC (fro). It is suitable to detect MAP4K5 in samples from Human.

Quick Overview for MAP4K5 antibody (AA 613-842) (ABIN7439981)

Target: MAP4K5 (Mitogen-Activated Protein Kinase Kinase Kinase Kinase 5 (MAP4K5))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAP4K5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-MAP4K5 Antibody

Binding Specificity: AA 613-842

Purpose: Polyclonal Antibody to Mitogen Activated Protein Kinase Kinase Kinase Kinase 5 (MAP4K5)

Specificity: The antibody is a rabbit polyclonal antibody raised against MAP4K5. It has been selected for its ability to recognize MAP4K5 in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Mitogen Activated Protein Kinase Kinase Kinase Kinase 5 (MAP4K5) corresdonding to Thr613~His842 (Accession # Q9Y4K4)

Isotype: IgG

Application Details

Application Notes: Western blotting: 1-5 μg/mLImmunocytochemistry in formalin fixed cells: 5-20 μg/mLImmunohistochemistry in formalin fixed frozen section: 5-20 μg/mLImmunohistochemistry in paraffin section: 5-20 μg/mLEnzyme-linked Immunosorbent Assay: 0.05-2 μg/mLOptimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for MAP4K5

Target: MAP4K5 (Mitogen-Activated Protein Kinase Kinase Kinase Kinase 5 (MAP4K5))

Alternative Name: MAP4K5

Background: MAP4-K5, GCKR, KHS, KHS1, MAPKKKK5, Germinal Center Kinase-Related, Kinase homologous to SPS1/STE20

UniProt: Q9Y4K4