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EpCAM antibody (AA 24-264)

This Rabbit Polyclonal antibody specifically detects EpCAM in WB, IHC, IP and ICC. It exhibits reactivity toward Human.
Catalog No. ABIN7433381

Quick Overview for EpCAM antibody (AA 24-264) (ABIN7433381)

Target

See all EpCAM (EPCAM) Antibodies
EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Reactivity

  • 373
  • 93
  • 60
  • 7
  • 2
  • 2
Human

Host

  • 213
  • 184
  • 13
  • 2
  • 1
  • 1
  • 1
Rabbit

Clonality

  • 283
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  • 3
Polyclonal

Conjugate

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  • 2
  • 2
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  • 2
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  • 2
  • 1
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  • 1
This EpCAM antibody is un-conjugated

Application

  • 266
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  • 196
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  • 95
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  • 1
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Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)
  • Binding Specificity

    • 92
    • 56
    • 33
    • 32
    • 29
    • 20
    • 17
    • 16
    • 16
    • 14
    • 12
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    • 1
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    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
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    AA 24-264

    Purpose

    Polyclonal Antibody to Epithelial Cell Adhesion Molecule (EPCAM)

    Specificity

    The antibody is a rabbit polyclonal antibody raised against EPCAM. It has been selected for its ability to recognize EPCAM in immunohistochemical staining and western blotting.

    Cross-Reactivity

    Pig

    Purification

    Antigen-specific affinity chromatography followed by Protein A affinity chromatography

    Immunogen

    Recombinant Epithelial Cell Adhesion Molecule (EPCAM) corresdonding to Gln24~Leu264 with N-terminal His and GST Tag

    Isotype

    IgG
  • Application Notes

    Western blotting: 0.01-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

    Comment

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    0.24 mg/mL

    Buffer

    0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

    Preservative

    ProClin, Sodium azide

    Precaution of Use

    This product contains ProClin and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

    Storage

    4 °C,-20 °C

    Storage Comment

    Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

    Expiry Date

    24 months
  • Target

    EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

    Alternative Name

    EPCAM

    Background

    CD326, TACSTD1, EGP, KSA, M4S1, MIC18, TROP1, HEGP2, ESA, Epithelial glycoprotein, Adenocarcinoma-associated antigen, Tumor Associated Calcium Signal Transducer 1

    UniProt

    P16422
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