EpCAM antibody (AA 25-265)

Catalog No. ABIN7443065

The Rabbit Polyclonal anti-EpCAM antibody has been validated for WB and IHC. It is suitable to detect EpCAM in samples from Pig.

Quick Overview for EpCAM antibody (AA 25-265) (ABIN7443065)

Target: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Reactivity: Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EpCAM antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-EpCAM Antibody

Binding Specificity: AA 25-265

Purpose: Polyclonal Antibody to Epithelial Cell Adhesion Molecule (EPCAM)

Specificity: The antibody is a rabbit polyclonal antibody raised against EPCAM. It has been selected for its ability to recognize EPCAM in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Epithelial Cell Adhesion Molecule (EPCAM) corresdonding to Gln25~Gln265 plus (LEHHHHHH) with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.01-3 μg/mL,Immunohistochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for EpCAM

Target: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Alternative Name: EPCAM

Background: CD326, TACSTD1, EGP, KSA, M4S1, MIC18, TROP1, HEGP2, ESA, Epithelial glycoprotein, Adenocarcinoma-associated antigen, Tumor Associated Calcium Signal Transducer 1

UniProt: Q75QW1