CD74 antibody (AA 65-277)

Catalog No. ABIN7443869

The Rabbit Polyclonal anti-CD74 antibody has been validated for WB, IHC, IP and ICC. It is suitable to detect CD74 in samples from Rat.

Quick Overview for CD74 antibody (AA 65-277) (ABIN7443869)

Target: CD74 (HLA-DR-gamma (CD74))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD74 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-CD74 Antibody

Binding Specificity: AA 65-277

Purpose: Polyclonal Antibody to Cluster Of Differentiation 74 (CD74)

Specificity: The antibody is a rabbit polyclonal antibody raised against CD74. It has been selected for its ability to recognize CD74 in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Cluster Of Differentiation 74 (CD74) corresdonding to Leu65~Gln277 with N-terminal His and GST Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,1:260-1060Immunohistochemistry: 5-20 μg/mL,1:26-106Immunocytochemistry: 5-20 μg/mL,1:26-106Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.53 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for CD74

Target: CD74 (HLA-DR-gamma (CD74))

Alternative Name: CD74

Background: MHCDG, HLA-DG, HLADG, DHLAG, Ia-GAMMA, HLA class II histocompatibility antigen gamma chain, Ia antigen-associated invariant chain, HLA-DR antigens-associated invariant chain

UniProt: P10247

Pathways: Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Negative Regulation of intrinsic apoptotic Signaling, Cancer Immune Checkpoints