LAG3 antibody (AA 209-451)

Catalog No. ABIN7443764

This Rabbit Polyclonal antibody specifically detects LAG3 in WB, IHC, IP and ICC. It exhibits reactivity toward Rat.

Quick Overview for LAG3 antibody (AA 209-451) (ABIN7443764)

Target: LAG3 (Lymphocyte-Activation Gene 3 (LAG3))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LAG3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-LAG3 Antibody

Binding Specificity: AA 209-451

Purpose: Polyclonal Antibody to Lymphocyte Activation Gene 3 (LAG3)

Specificity: The antibody is a rabbit polyclonal antibody raised against LAG3. It has been selected for its ability to recognize LAG3 in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Lymphocyte Activation Gene 3 (LAG3) corresdonding to Asn209~Leu451

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,1:500-2000Immunohistochemistry: 5-20 μg/mL,1:50-200Immunocytochemistry: 5-20 μg/mL,1:50-200Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.98 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for LAG3

Target: LAG3 (Lymphocyte-Activation Gene 3 (LAG3))

Alternative Name: LAG3

Background: CD223, Protein FDC

UniProt: Q5BK54

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints