MCAM antibody (AA 324-565)

Catalog No. ABIN7433909

This Rabbit Polyclonal antibody specifically detects MCAM in WB, IF and ICC. It exhibits reactivity toward Human.

Quick Overview for MCAM antibody (AA 324-565) (ABIN7433909)

Target: MCAM (Melanoma Cell Adhesion Molecule (MCAM))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MCAM antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-MCAM Antibody

Binding Specificity: AA 324-565

Purpose: Polyclonal Antibody to Cluster Of Differentiation 146 (CD146)

Specificity: The antibody is a rabbit polyclonal antibody raised against CD146. It has been selected for its ability to recognize CD146 in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Cluster Of Differentiation 146 (CD146) corresdonding to Asp324~Val565 (Accession # P43121)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.01-2 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for MCAM

Target: MCAM (Melanoma Cell Adhesion Molecule (MCAM))

Alternative Name: CD146

Background: MCAM, MUC18, Melanoma Cell Adhesion Molecule, Cell surface glycoprotein P1H12, Melanoma-associated antigen A32, S-endo 1 endothelial-associated antigen

UniProt: P43121