NFKBIB antibody (AA 85-332)

Catalog No. ABIN7442534

The Rabbit Polyclonal anti-NFKBIB antibody has been validated for WB, ELISA, IHC (p), ICC and IHC (fro). It is suitable to detect NFKBIB in samples from Human.

Quick Overview for NFKBIB antibody (AA 85-332) (ABIN7442534)

Target: NFKBIB (NF-kappa-B inhibitor beta (NFKBIB))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NFKBIB antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-NFKBIB Antibody

Binding Specificity: AA 85-332

Purpose: Polyclonal Antibody to Inhibitory Subunit Of NF Kappa B Beta (IkBb)

Specificity: The antibody is a rabbit polyclonal antibody raised against IkBb. It has been selected for its ability to recognize IkBb in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Inhibitory Subunit Of NF Kappa B Beta (IkBb) corresdonding to Glu85~His332 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,1:350-1300,Immunohistochemistry: 5-20 μg/mL,1:35-130,Immunocytochemistry: 5-20 μg/mL,1:35-130,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for NFKBIB

Target: NFKBIB (NF-kappa-B inhibitor beta (NFKBIB))

Alternative Name: IkBb

Background: TRIP9, NFKBIB, Thyroid receptor-interacting protein 9, Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells Inhibitor,Beta, NF-Kappa-B Inhibitor Beta

UniProt: Q15653

Pathways: NF-kappaB Signaling, Activation of Innate immune Response, Maintenance of Protein Location, Toll-Like Receptors Cascades