NFKBIB antibody (AA 85-332)

Catalog No. ABIN7442534

Product Details anti-NFKBIB Antibody

Target: NFKBIB (NF-kappa-B inhibitor beta (NFKBIB))

Binding Specificity: AA 85-332

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NFKBIB antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Purpose: Polyclonal Antibody to Inhibitory Subunit Of NF Kappa B Beta (IkBb)

Specificity: The antibody is a rabbit polyclonal antibody raised against IkBb. It has been selected for its ability to recognize IkBb in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Inhibitory Subunit Of NF Kappa B Beta (IkBb) corresdonding to Glu85~His332 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,1:350-1300,Immunohistochemistry: 5-20 μg/mL,1:35-130,Immunocytochemistry: 5-20 μg/mL,1:35-130,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for NFKBIB

Target: NFKBIB (NF-kappa-B inhibitor beta (NFKBIB))

Alternative Name: IkBb (NFKBIB Products)

Background: TRIP9, NFKBIB, Thyroid receptor-interacting protein 9, Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells Inhibitor,Beta, NF-Kappa-B Inhibitor Beta

UniProt: Q15653

Pathways: NF-kappaB Signaling, Activation of Innate immune Response, Maintenance of Protein Location, Toll-Like Receptors Cascades