CHRNA4 antibody (AA 49-242)

Catalog No. ABIN7444463

The Rabbit Polyclonal anti-CHRNA4 antibody has been validated for WB, ELISA, ICC, IHC (p) and IHC (fro). It is suitable to detect CHRNA4 in samples from Human.

Quick Overview for CHRNA4 antibody (AA 49-242) (ABIN7444463)

Target: CHRNA4 (Cholinergic Receptor, Nicotinic, alpha 4 (CHRNA4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CHRNA4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-CHRNA4 Antibody

Binding Specificity: AA 49-242

Purpose: Polyclonal Antibody to Cholinergic Receptor, Nicotinic, Alpha 4 (CHRNa4)

Specificity: The antibody is a rabbit polyclonal antibody raised against CHRNa4. It has been selected for its ability to recognize CHRNa4 in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Cholinergic Receptor, Nicotinic, Alpha 4 (CHRNa4) corresdonding to Asn49~Leu242 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,1:500-2000,Immunohistochemistry: 5-20 μg/mL,1:50-200,Immunocytochemistry: 5-20 μg/mL,1:50-200,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for CHRNA4

Target: CHRNA4 (Cholinergic Receptor, Nicotinic, alpha 4 (CHRNA4))

Alternative Name: CHRNa4

Background: CHR-NA4, CHRN-A4, BFNC, EBN, EBN1, NACRA4, N-AChRA4, NAChRA4, N-AChR-A4, Neuronal Acetylcholine Receptor Alpha 4

UniProt: P43681

Pathways: Synaptic Membrane