SMAD4 antibody (AA 9-231)

Catalog No. ABIN7441925

This Rabbit Polyclonal antibody specifically detects SMAD4 in WB, IHC, IP and ICC. It exhibits reactivity toward Mouse.

Quick Overview for SMAD4 antibody (AA 9-231) (ABIN7441925)

Target: SMAD4 (SMAD Family Member 4 (SMAD4))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMAD4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-SMAD4 Antibody

Binding Specificity: AA 9-231

Purpose: Polyclonal Antibody to Mothers Against Decapentaplegic Homolog 4 (Smad4)

Specificity: The antibody is a rabbit polyclonal antibody raised against Smad4. It has been selected for its ability to recognize Smad4 in immunohistochemical staining and western blotting.

Cross-Reactivity: Human

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Mothers Against Decapentaplegic Homolog 4 (Smad4) corresdonding to Thr9~Ser231 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for SMAD4

Target: SMAD4 (SMAD Family Member 4 (SMAD4))

Alternative Name: Smad4

Background: DPC4, JIP, MADH4, Deletion target in pancreatic carcinoma 4

UniProt: P97471

Pathways: Cell Division Cycle, Chromatin Binding, Autophagy