TNC antibody (AA 174-621)

Catalog No. ABIN7443281

The Rabbit Polyclonal anti-TNC antibody has been validated for WB, IHC, IP and ICC. It is suitable to detect TNC in samples from Mouse.

Quick Overview for TNC antibody (AA 174-621) (ABIN7443281)

Target: TNC (Tenascin C (TNC))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TNC antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-TNC Antibody

Binding Specificity: AA 174-621

Purpose: Polyclonal Antibody to Tenascin C (TNC)

Specificity: The antibody is a rabbit polyclonal antibody raised against TNC. It has been selected for its ability to recognize TNC in immunohistochemical staining and western blotting.

Cross-Reactivity: Human

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Tenascin C (TNC) corresdonding to Cys174~Ser621 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.01-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin, Sodium azide

Precaution of Use: This product contains ProClin and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for TNC

Target: TNC (Tenascin C (TNC))

Alternative Name: Tenascin C

Background: TN-C, HXB, TN, GMEM, JI, Myotendinous antigen, Neuronectin, GP 150-225, Cytotactin, Hexabrachion, Glioma-associated-extracellular matrix antigen

UniProt: Q80YX1

Pathways: Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development