SMAD2 antibody (AA 272-467)

Catalog No. ABIN7434686

The Rabbit Polyclonal anti-SMAD2 antibody has been validated for WB, IHC, IP and ICC. It is suitable to detect SMAD2 in samples from Mouse.

Quick Overview for SMAD2 antibody (AA 272-467) (ABIN7434686)

Target: SMAD2 (SMAD, Mothers Against DPP Homolog 2 (SMAD2))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMAD2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-SMAD2 Antibody

Binding Specificity: AA 272-467

Purpose: Polyclonal Antibody to Mothers Against Decapentaplegic Homolog 2 (Smad2)

Specificity: The antibody is a rabbit polyclonal antibody raised against Smad2. It has been selected for its ability to recognize Smad2 in immunohistochemical staining and western blotting.

Cross-Reactivity: Human, Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Mothers Against Decapentaplegic Homolog 2 (Smad2) corresdonding to Ala272~Ser467 (Accession # Q62432)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.59 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for SMAD2

Target: SMAD2 (SMAD, Mothers Against DPP Homolog 2 (SMAD2))

Alternative Name: Smad2

Background: JV18, JV18-1, MADH2, MADR2, HMAD-2, HSMAD2, Mad-related protein 2

UniProt: Q62432

Pathways: Cell Division Cycle, Hormone Transport, Chromatin Binding, Protein targeting to Nucleus