LEFTY1 antibody (AA 169-368)

Catalog No. ABIN7438841

This Rabbit Polyclonal antibody specifically detects LEFTY1 in WB, IHC, IP and ICC. It exhibits reactivity toward Mouse.

Quick Overview for LEFTY1 antibody (AA 169-368) (ABIN7438841)

Target: LEFTY1 (Left-Right Determination Factor 1 (LEFTY1))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LEFTY1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-LEFTY1 Antibody

Binding Specificity: AA 169-368

Purpose: Polyclonal Antibody to Left/Right Determination Factor 1 (LEFTY1)

Specificity: The antibody is a rabbit polyclonal antibody raised against LEFTY1. It has been selected for its ability to recognize LEFTY1 in immunohistochemical staining and western blotting.

Cross-Reactivity: Human, Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Left/Right Determination Factor 1 (LEFTY1) corresdonding to Ser169~Pro368 with N-terminal His and GST Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mLImmunocytochemistry in formalin fixed cells: 5-20 μg/mLImmunohistochemistry in formalin fixed frozen section: 5-20 μg/mLImmunohistochemistry in paraffin section: 5-20 μg/mLEnzyme-linked Immunosorbent Assay: 0.05-2 μg/mLOptimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for LEFTY1

Target: LEFTY1 (Left-Right Determination Factor 1 (LEFTY1))

Alternative Name: LEFTY1

Background: LEFTY-B, LEFTYB, LEFT-B, LEFTB, Left Right Determination Factor B

UniProt: Q64280