alpha Actinin 4 antibody (AA 1-269)

Catalog No. ABIN7440797

This Rabbit Polyclonal antibody specifically detects alpha Actinin 4 in WB and IHC. It exhibits reactivity toward Human.

Quick Overview for alpha Actinin 4 antibody (AA 1-269) (ABIN7440797)

Target: alpha Actinin 4 (ACTN4) (Actinin, alpha 4 (ACTN4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This alpha Actinin 4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-alpha Actinin 4 Antibody

Binding Specificity: AA 1-269

Purpose: Polyclonal Antibody to Actinin Alpha 4 (ACTN4)

Specificity: The antibody is a rabbit polyclonal antibody raised against ACTN4. It has been selected for its ability to recognize ACTN4 in immunohistochemical staining and western blotting.

Cross-Reactivity: Pig

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Actinin Alpha 4 (ACTN4) corresdonding to Met1~Ser269 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.01-3 μg/mL,Immunohistochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for alpha Actinin 4

Target: alpha Actinin 4 (ACTN4) (Actinin, alpha 4 (ACTN4))

Alternative Name: ACTN4

Background: FSGS, FSGS1, F-actin cross-linking protein, Non-muscle alpha-actinin 4

UniProt: O43707

Pathways: Proton Transport