LGMN antibody (AA 18-433)

Catalog No. ABIN7435007

This Rabbit Polyclonal antibody specifically detects LGMN in WB, IHC, IP and ICC. It exhibits reactivity toward Human.

Quick Overview for LGMN antibody (AA 18-433) (ABIN7435007)

Target: LGMN (Legumain (LGMN))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LGMN antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-LGMN Antibody

Binding Specificity: AA 18-433

Purpose: Polyclonal Antibody to Legumain (LGMN)

Specificity: The antibody is a rabbit polyclonal antibody raised against LGMN. It has been selected for its ability to recognize LGMN in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse, Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Legumain (LGMN) corresdonding to Val18~Tyr433 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.01-3 μg/mL,Immunohistochemistry: 5-30 μg/mL,Immunocytochemistry: 5-30 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin, Sodium azide

Precaution of Use: This product contains ProClin and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for LGMN

Target: LGMN (Legumain (LGMN))

Alternative Name: LGMN

Background: AEP, LGMN1, PRSC1, Protease,Cysteine 1, Asparaginyl endopeptidase

UniProt: Q99538

Pathways: Metabolism of Steroid Hormones and Vitamin D, Activation of Innate immune Response, Toll-Like Receptors Cascades