LIM2 antibody (AA 30-136)

Catalog No. ABIN7444232

The Rabbit Polyclonal anti-LIM2 antibody (ABIN7444232) specifically detects LIM2 in WB, IHC, IP and ICC. The antibody is reactive with Human samples.

Quick Overview for LIM2 antibody (AA 30-136) (ABIN7444232)

Target: LIM2 (Lens Intrinsic Membrane Protein 2, 19kDa (LIM2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LIM2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-LIM2 Antibody

Binding Specificity: AA 30-136

Purpose: Polyclonal Antibody to Lens Intrinsic Membrane Protein 2 (LIM2)

Specificity: The antibody is a rabbit polyclonal antibody raised against LIM2. It has been selected for its ability to recognize LIM2 in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse, Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Lens Intrinsic Membrane Protein 2 (LIM2) corresdonding to Gln30~Phe136 with Two N-terminal Tags, His-tag and SUMO-tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,1:480-1900
Immunohistochemistry: 5-20 μg/mL,1:48-190
Immunocytochemistry: 5-20 μg/mL,1:48-190
Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.97 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for LIM2

Target: LIM2 (Lens Intrinsic Membrane Protein 2, 19kDa (LIM2))

Alternative Name: LIM2

Background: MP19, Lens Fiber Membrane Intrinsic Protein

UniProt: P55344

Pathways: Cell-Cell Junction Organization