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PI3 antibody (AA 41-117)

The Rabbit Polyclonal anti-PI3 antibody has been validated for WB, IHC, IP and ICC. It is suitable to detect PI3 in samples from Human.
Catalog No. ABIN7435181

Quick Overview for PI3 antibody (AA 41-117) (ABIN7435181)

Target

See all PI3 Antibodies
PI3 (Peptidase Inhibitor 3, Skin-Derived (PI3))

Reactivity

  • 53
  • 2
  • 2
  • 1
  • 1
  • 1
Human

Host

  • 32
  • 19
  • 1
  • 1
Rabbit

Clonality

  • 34
  • 19
Polyclonal

Conjugate

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  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This PI3 antibody is un-conjugated

Application

  • 50
  • 19
  • 14
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  • 11
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  • 8
  • 3
  • 2
  • 2
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Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)
  • Binding Specificity

    • 17
    • 8
    • 3
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    • 1
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    AA 41-117

    Purpose

    Polyclonal Antibody to Peptidase Inhibitor 3, Skin Derived (PI3)

    Specificity

    The antibody is a rabbit polyclonal antibody raised against PI3. It has been selected for its ability to recognize PI3 in immunohistochemical staining and western blotting.

    Purification

    Antigen-specific affinity chromatography followed by Protein A affinity chromatography

    Immunogen

    Recombinant Peptidase Inhibitor 3, Skin Derived (PI3) corresdonding to Phe41~Gln117 with N-terminal His Tag

    Isotype

    IgG
  • Application Notes

    Western blotting: 0.5-2 μg/mL,Immunocytochemistry in formalin fixed cells: 5-20 μg/mL,Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL,Immunohistochemistry in paraffin section: 5-20 μg/mL,Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL,Optimal working dilutions must be determined by end user.

    Comment

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    500 μg/mL

    Buffer

    PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    4 °C,-20 °C

    Storage Comment

    Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

    Expiry Date

    24 months
  • Target

    PI3 (Peptidase Inhibitor 3, Skin-Derived (PI3))

    Alternative Name

    Peptidase Inhibitor 3, Skin Derived

    Background

    SKALP, ESI, WAP3, WFDC14, Elafin, Skin-Derived Antileukoproteinase, Cementoin, Elastase-specific inhibitor, WAP four-disulfide core domain protein 14

    UniProt

    P19957
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