PDGFB antibody (AA 84-199)

Catalog No. ABIN7435253

The Rabbit Polyclonal anti-PDGFB antibody (ABIN7435253) specifically detects PDGFB in WB, IHC, IP and ICC. The antibody is reactive with Pig samples.

Quick Overview for PDGFB antibody (AA 84-199) (ABIN7435253)

Target: PDGFB (Platelet Derived Growth Factor Subunit B (PDGFB))

Reactivity: Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PDGFB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-PDGFB Antibody

Binding Specificity: AA 84-199

Purpose: Polyclonal Antibody to Platelet Derived Growth Factor Subunit B (PDGFB)

Specificity: The antibody is a rabbit polyclonal antibody raised against PDGFB. It has been selected for its ability to recognize PDGFB in immunohistochemical staining and western blotting.

Cross-Reactivity: Human

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Platelet Derived Growth Factor Subunit B (PDGFB) corresdonding to RAGCLSLRVGVGPSASAALDGPPLPTLTEFPLPP+Gly84~Arg199+GNHSSRVC with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for PDGFB

Target: PDGFB (Platelet Derived Growth Factor Subunit B (PDGFB))

Alternative Name: PDGFB

Background: PDGF-B, PDGF2, SIS, SSV, C-sis, Simian Sarcoma Viral(v-sis)Oncogene Homolog, Platelet Derived Growth Factor Beta Polypeptide, Proto-oncogene c-Sis, Becaplermin

UniProt: A0A287ADF0

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling