Intrinsic Factor antibody (AA 31-288)

Catalog No. ABIN7443227

This Rabbit Polyclonal antibody specifically detects Intrinsic Factor in WB, IHC, IP and ICC. It exhibits reactivity toward Mouse.

Quick Overview for Intrinsic Factor antibody (AA 31-288) (ABIN7443227)

Target: Intrinsic Factor (GIF) (Gastric Intrinsic Factor (Vitamin B Synthesis) (GIF))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Intrinsic Factor antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-Intrinsic Factor Antibody

Binding Specificity: AA 31-288

Purpose: Polyclonal Antibody to Gastric Intrinsic Factor (GIF)

Specificity: The antibody is a rabbit polyclonal antibody raised against GIF. It has been selected for its ability to recognize GIF in immunohistochemical staining and western blotting.

Cross-Reactivity: Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Gastric Intrinsic Factor (GIF) corresdonding to Asp31~Cys288 (Accession # P52787)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.01-3 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for Intrinsic Factor

Target: Intrinsic Factor (GIF) (Gastric Intrinsic Factor (Vitamin B Synthesis) (GIF))

Alternative Name: Gastric Intrinsic Factor

Background: TCN3, IF, IFMH, INF, Transcobalamin III, Vitamin B Synthesis

UniProt: P52787