Intrinsic Factor antibody (AA 196-414)

Catalog No. ABIN7441955

The Rabbit Polyclonal anti-Intrinsic Factor antibody has been validated for WB, IHC, IP and ICC. It is suitable to detect Intrinsic Factor in samples from Rat.

Quick Overview for Intrinsic Factor antibody (AA 196-414) (ABIN7441955)

Target: Intrinsic Factor (GIF) (Gastric Intrinsic Factor (Vitamin B Synthesis) (GIF))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Intrinsic Factor antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-Intrinsic Factor Antibody

Binding Specificity: AA 196-414

Purpose: Polyclonal Antibody to Gastric Intrinsic Factor (GIF)

Specificity: The antibody is a rabbit polyclonal antibody raised against GIF. It has been selected for its ability to recognize GIF in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Gastric Intrinsic Factor (GIF) corresdonding to Arg196~Phe414

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for Intrinsic Factor

Target: Intrinsic Factor (GIF) (Gastric Intrinsic Factor (Vitamin B Synthesis) (GIF))

Alternative Name: Gastric Intrinsic Factor

Background: TCN3, IF, IFMH, INF, Transcobalamin III, Vitamin B Synthesis

UniProt: P17267