Indian Hedgehog antibody (AA 45-195)

Catalog No. ABIN7445750

The Rabbit Polyclonal anti-Indian Hedgehog antibody has been validated for WB, IHC, IP and ICC. It is suitable to detect Indian Hedgehog in samples from Human.

Quick Overview for Indian Hedgehog antibody (AA 45-195) (ABIN7445750)

Target: Indian Hedgehog (IHH)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Indian Hedgehog antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-Indian Hedgehog Antibody

Binding Specificity: AA 45-195

Purpose: Polyclonal Antibody to Hedgehog Homolog, Indian (IHH)

Specificity: The antibody is a rabbit polyclonal antibody raised against IHH. It has been selected for its ability to recognize IHH in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Hedgehog Homolog, Indian (IHH) corresdonding to Val45~Ser195

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,1:500-2000,Immunohistochemistry: 5-20 μg/mL,1:50-200,Immunocytochemistry: 5-20 μg/mL,1:50-200,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for Indian Hedgehog

Target: Indian Hedgehog (IHH)

Alternative Name: Hedgehog Homolog, Indian

Background: BDA1, HHG2

UniProt: Q14623

Pathways: Hedgehog Signaling