PRKAA2 antibody (AA 252-493)

Catalog No. ABIN7443308

The Rabbit Polyclonal anti-PRKAA2 antibody is suitable to detect PRKAA2 in samples from Human. It has been validated for WB, ELISA, IHC (p), ICC and IHC (fro).

Quick Overview for PRKAA2 antibody (AA 252-493) (ABIN7443308)

Target: PRKAA2 (Protein Kinase, AMP-Activated, alpha 2 Catalytic Subunit (PRKAA2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRKAA2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-PRKAA2 Antibody

Binding Specificity: AA 252-493

Purpose: Polyclonal Antibody to Protein Kinase, AMP Activated Alpha 2 (PRKAa2)

Specificity: The antibody is a rabbit polyclonal antibody raised against PRKAa2. It has been selected for its ability to recognize PRKAa2 in immunohistochemical staining and western blotting.

Cross-Reactivity: Chicken, Chinese Hamster, Cow, Dog, Goat, Mouse, Pig, Rat, Sheep

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Protein Kinase, AMP Activated Alpha 2 (PRKAa2) corresdonding to Asp252~Ala493 (Accession # P54646)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.01-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for PRKAA2

Target: PRKAA2 (Protein Kinase, AMP-Activated, alpha 2 Catalytic Subunit (PRKAA2))

Alternative Name: PRKAa2

Background: AMPK, AMPK2, PRKAA, 5'-AMP-Activated Protein Kinase Catalytic Subunit Alpha-2, Acetyl-CoA carboxylase kinase, Hydroxymethylglutaryl-CoA reductase kinase

UniProt: P54646

Pathways: AMPK Signaling, Carbohydrate Homeostasis, Chromatin Binding, Regulation of Carbohydrate Metabolic Process, Warburg Effect