EPAS1 antibody (AA 26-347)

Catalog No. ABIN7445813

The Rabbit Polyclonal anti-EPAS1 antibody has been validated for WB, IHC, IP and ICC. It is suitable to detect EPAS1 in samples from Human.

Quick Overview for EPAS1 antibody (AA 26-347) (ABIN7445813)

Target: EPAS1 (Endothelial PAS Domain Protein 1 (EPAS1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EPAS1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-EPAS1 Antibody

Binding Specificity: AA 26-347

Purpose: Polyclonal Antibody to Hypoxia Inducible Factor 2 Alpha (HIF2a)

Specificity: The antibody is a rabbit polyclonal antibody raised against HIF2a. It has been selected for its ability to recognize HIF2a in immunohistochemical staining and western blotting.

Cross-Reactivity: Pig

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Hypoxia Inducible Factor 2 Alpha (HIF2a) corresdonding to Lys26~Ile347 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for EPAS1

Target: EPAS1 (Endothelial PAS Domain Protein 1 (EPAS1))

Alternative Name: HIF2a

Background: EPAS1, HLF, MOP2, PASD2, BHLHE73, Endothelial PAS Domain 1, HIF-1 Alpha-Like Factor, Basic-helix-loop-helix-PAS protein MOP2, Class E basic helix-loop-helix protein 73

UniProt: Q99814

Pathways: Signaling Events mediated by VEGFR1 and VEGFR2, Warburg Effect