DEFA1B antibody (AA 20-94)

Catalog No. ABIN7441754

This Rabbit Polyclonal antibody specifically detects DEFA1B in WB, ICC, ELISA, IHC (fro) and IHC (p). It exhibits reactivity toward Human.

Quick Overview for DEFA1B antibody (AA 20-94) (ABIN7441754)

Target: DEFA1B (Defensin, alpha 1B (DEFA1B))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DEFA1B antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-DEFA1B Antibody

Binding Specificity: AA 20-94

Purpose: Polyclonal Antibody to Defensin Alpha 1B (DEFa1B)

Specificity: The antibody is a rabbit polyclonal antibody raised against DEFa1B. It has been selected for its ability to recognize DEFa1B in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse, Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Defensin Alpha 1B (DEFa1B) corresdonding to Glu20~Cys94 with N-terminal His and GST Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 1-5 μg/mLImmunocytochemistry in formalin fixed cells: 5-20 μg/mLImmunohistochemistry in formalin fixed frozen section: 5-20 μg/mLImmunohistochemistry in paraffin section: 5-20 μg/mLEnzyme-linked Immunosorbent Assay: 0.05-2 μg/mLOptimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for DEFA1B

Target: DEFA1B (Defensin, alpha 1B (DEFA1B))

Alternative Name: DEFa1B

Background: HNP-1, DEFA2, MRS, Neutrophil defensin 1

UniProt: P59665