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Elastase 3B antibody (AA 28-267)

The Rabbit Polyclonal anti-Elastase 3B antibody has been validated for WB, IHC, IP and ICC. It is suitable to detect Elastase 3B in samples from Mouse.
Catalog No. ABIN7437587

Quick Overview for Elastase 3B antibody (AA 28-267) (ABIN7437587)

Target

See all Elastase 3B (ELA3B) Antibodies
Elastase 3B (ELA3B) (Elastase 3B, Pancreatic (ELA3B))

Reactivity

  • 123
  • 25
  • 21
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Mouse

Host

  • 80
  • 57
Rabbit

Clonality

  • 104
  • 33
Polyclonal

Conjugate

  • 55
  • 21
  • 9
  • 8
  • 8
  • 8
  • 8
  • 8
  • 8
  • 1
  • 1
  • 1
  • 1
This Elastase 3B antibody is un-conjugated

Application

  • 81
  • 66
  • 55
  • 42
  • 37
  • 36
  • 32
  • 14
  • 10
  • 8
  • 5
  • 4
  • 4
Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)
  • Binding Specificity

    • 96
    • 9
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 28-267

    Purpose

    Polyclonal Antibody to Elastase 3B (ELA3B)

    Specificity

    The antibody is a rabbit polyclonal antibody raised against ELA3B. It has been selected for its ability to recognize ELA3B in immunohistochemical staining and western blotting.

    Cross-Reactivity

    Human, Rat

    Purification

    Antigen-specific affinity chromatography followed by Protein A affinity chromatography

    Immunogen

    Recombinant Elastase 3B (ELA3B) corresdonding to Val28~Ala267 with N-terminal His Tag

    Isotype

    IgG
  • Application Notes

    Western blotting: 0.01-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

    Comment

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    0.48 mg/mL

    Buffer

    PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

    Preservative

    ProClin, Sodium azide

    Precaution of Use

    This product contains ProClin and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

    Storage

    4 °C,-20 °C

    Storage Comment

    Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

    Expiry Date

    24 months
  • Target

    Elastase 3B (ELA3B) (Elastase 3B, Pancreatic (ELA3B))

    Alternative Name

    ELA3B

    Background

    CELA3B, Chymotrypsin Like Elastase Family Member 3B, Protease E, Fecal Elastase, Elastase 1, Cholesterol-Binding Pancreatic Protease, Pancreatic Endopeptidase E

    UniProt

    Q9CQ52
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