UBE2L3 antibody (AA 4-154)

Catalog No. ABIN7441196

The Rabbit Polyclonal anti-UBE2L3 antibody has been validated for WB, IHC, IP and ICC. It is suitable to detect UBE2L3 in samples from Human.

Quick Overview for UBE2L3 antibody (AA 4-154) (ABIN7441196)

Target: UBE2L3 (Ubiquitin-Conjugating Enzyme E2L 3 (UBE2L3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBE2L3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-UBE2L3 Antibody

Binding Specificity: AA 4-154

Purpose: Polyclonal Antibody to Ubiquitin Conjugating Enzyme E2L3 (UBE2L3)

Specificity: The antibody is a rabbit polyclonal antibody raised against UBE2L3. It has been selected for its ability to recognize UBE2L3 in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse, Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Ubiquitin Conjugating Enzyme E2L3 (UBE2L3) corresdonding to Ser4~Asp154 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,1:250-1000Immunohistochemistry: 5-20 μg/mL,1:25-100Immunocytochemistry: 5-20 μg/mL,1:25-100Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for UBE2L3

Target: UBE2L3 (Ubiquitin-Conjugating Enzyme E2L 3 (UBE2L3))

Alternative Name: UBE2L3

Background: L-UBC, E2-F1, UBCH7, UbcM4, Ubiquitin carrier protein L3, Ubiquitin-conjugating enzyme E2-F1, Ubiquitin-protein ligase L3

UniProt: P68036