UCP1 antibody (AA 179-296)

Catalog No. ABIN7436075

The Rabbit Polyclonal anti-UCP1 antibody has been validated for WB, IHC, IP and ICC. It is suitable to detect UCP1 in samples from Mouse.

Quick Overview for UCP1 antibody (AA 179-296) (ABIN7436075)

Target: UCP1 (Uncoupling Protein 1 (Mitochondrial, Proton Carrier) (UCP1))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UCP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-UCP1 Antibody

Binding Specificity: AA 179-296

Purpose: Polyclonal Antibody to Uncoupling Protein 1, Mitochondrial (UCP1)

Specificity: The antibody is a rabbit polyclonal antibody raised against UCP1. It has been selected for its ability to recognize UCP1 in immunohistochemical staining and western blotting.

Cross-Reactivity: Human

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Uncoupling Protein 1, Mitochondrial (UCP1) corresdonding to Pro179~Leu296 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.01-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for UCP1

Target: UCP1 (Uncoupling Protein 1 (Mitochondrial, Proton Carrier) (UCP1))

Alternative Name: UCP1

Background: SLC25A7, UCP, Thermogenin, Solute carrier family 25 member 7

UniProt: P12242

Pathways: Proton Transport, Brown Fat Cell Differentiation